5JYL
Human P-cadherin MEC1 with scFv TSP7 bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.570, 49.410, 111.660 |
| Unit cell angles | 90.00, 101.89, 90.00 |
Refinement procedure
| Resolution | 109.260 - 2.550 |
| R-factor | 0.21693 |
| Rwork | 0.214 |
| R-free | 0.27090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qok |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.291 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.260 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.125 | 0.670 |
| Number of reflections | 23310 | |
| <I/σ(I)> | 10.4 | 2.4 |
| Completeness [%] | 99.1 | 96.3 |
| Redundancy | 5.4 | 5.2 |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM potassium phosphate monobasic, 20% PEG 3350 |
