5JYI
Trypsin bound with succinic acid at 1.9A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-08-14 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.320, 53.180, 76.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.405 - 1.914 |
R-factor | 0.1576 |
Rwork | 0.153 |
R-free | 0.20140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i8h |
RMSD bond length | 0.008 |
RMSD bond angle | 1.164 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.660 |
High resolution limit [Å] | 1.900 |
Number of reflections | 15134 |
<I/σ(I)> | 19 |
Completeness [%] | 99.3 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.5 | 298.15 | 0.1M Succinic acid,0.1M Sodium Acetate ph 5.8,15%(w/v) PEG3350 |