5JXL
Cryo-EM structure of the flagellar hook of Campylobacter jejuni
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.5 |
| Number of Particle | 70477 |
| Software | e2helixboxer.py,segclassreconstruct,segrefine3d,segmentclass,PHENIX,Leginon,ctffind3,CTFTILT,Coot,O |
| CTF correction | PHASE FLIPPING ONLY |
