5JXD

Crystal structure of murine Tnfaip8 C165S mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97933
Spacegroup name	I 2 2 2
Unit cell lengths	66.276, 71.570, 90.596
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.138 - 2.029
R-factor	0.2395
Rwork	0.237
R-free	0.28690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f4m
RMSD bond length	0.008
RMSD bond angle	1.702
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.140	2.070
High resolution limit [Å]	2.029	2.030
Rmerge	0.078	0.869
Number of reflections	13998
<I/σ(I)>	23.15	1.58
Completeness [%]	97.4	74.8
Redundancy	6.7	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	296	0.1M Bis-Tris propane, pH 7.7, 1.2M sodium citrate