5JXC

SynGAP Coiled-coil trimer

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97886
Spacegroup name	P 1 21 1
Unit cell lengths	28.803, 236.664, 42.207
Unit cell angles	90.00, 96.92, 90.00

Refinement procedure

Resolution	39.497 - 2.500
R-factor	0.2271
Rwork	0.223
R-free	0.30700
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	0.969
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	19240
<I/σ(I)>	22.8
Completeness [%]	99.7
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1M HEPES sodium pH 7.5, 0.1M L-proline, 20% w/v Polyethylene glycol 1500