5JXB
PSD-95 extended PDZ3 in complex with SynGAP PBM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97950 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 70.286, 71.094, 115.791 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.890 - 2.900 |
R-factor | 0.2411 |
Rwork | 0.241 |
R-free | 0.28740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1be9 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.867 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 6680 |
<I/σ(I)> | 43.3 |
Completeness [%] | 99.2 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M HEPES sodium pH 7.5, 10% v/v 2-Propanol, 20% w/v Polyethylene glycol 4000 |