5JX4

Crystal structure of E36-G37del mutant of the Bacillus caldolyticus cold shock protein.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-08-21
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	34.009, 44.077, 36.484
Unit cell angles	90.00, 99.28, 90.00

Refinement procedure

Resolution	18.797 - 1.800
R-factor	0.1515
Rwork	0.150
R-free	0.18210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hax
RMSD bond length	0.013
RMSD bond angle	1.335
Data reduction software	XDS
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.800	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.048	0.114
Number of reflections	9547
<I/σ(I)>	23.6
Completeness [%]	95.6	70.3
Redundancy	3.5	2.5
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	0.1 M Sodium Acetate, Poly(ethylene glycol) methyl ether 2000 30%, 0.2 M Ammonium Sulfate. Protein 20 mg/mL in 20 mM Sodium phosphate.