Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 117.769, 134.065, 139.101 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.240 - 2.300 |
| R-factor | 0.21688 |
| Rwork | 0.215 |
| R-free | 0.24853 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4DOF_A |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.223 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.240 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.253 |
| Number of reflections | 98092 | |
| <I/σ(I)> | 10.4 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.48 | 7.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 5% PEG3000, 0.1M NaCl, 0.1M Hepes |
