5JWZ
Structure of a Putative Xyloglucanase from the Cellulolytic Bacteria Streptomyces sp. SirexAA-E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 104.149, 140.181, 116.711 |
Unit cell angles | 90.00, 113.92, 90.00 |
Refinement procedure
Resolution | 29.887 - 1.695 |
R-factor | 0.1281 |
Rwork | 0.128 |
R-free | 0.16880 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.127 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.738 |
High resolution limit [Å] | 1.695 | 1.695 |
Rmerge | 0.102 | 0.781 |
Number of reflections | 159374 | |
<I/σ(I)> | 24.67 | 3.1 |
Completeness [%] | 94.4 | 70 |
Redundancy | 13 | 12.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 20% PEG 6K, 200mM Calcium Chloride, and 100mM of HEPES buffered at pH 7.0 |