5JVU
The neck-linker and alpha 7 helix of Drosophila melanogaster kinesin-1 fused to EB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.973, 37.783, 52.706 |
| Unit cell angles | 90.00, 114.96, 90.00 |
Refinement procedure
| Resolution | 28.263 - 1.948 |
| R-factor | 0.2001 |
| Rwork | 0.198 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yib |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.793 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.263 | 2.072 |
| High resolution limit [Å] | 1.948 | 1.948 |
| Rmerge | 0.083 | 0.589 |
| Number of reflections | 12095 | |
| <I/σ(I)> | 16.6 | 2.1 |
| Completeness [%] | 99.0 | 97.3 |
| Redundancy | 4.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20% (w/v) methoxy polyethylene glycol (MEPEG) 5000, 100 mM Li2SO4, 100 mM MES/Acetate pH 5.5, 10 mM gamma-caprolactone |
