5JVS

The neck-linker + DAL and alpha 7 helix of Drosophila melanogaster Kinesin-1 fused to EB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97934
Spacegroup name	I 41 2 2
Unit cell lengths	40.016, 40.016, 185.369
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.588 - 2.248
R-factor	0.202
Rwork	0.200
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yib
RMSD bond length	0.002
RMSD bond angle	0.330
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.340	2.280
High resolution limit [Å]	2.240	2.240
Rmerge	0.058	0.522
Number of reflections	5804
<I/σ(I)>	37.5	4.6
Completeness [%]	90.0	69.5
Redundancy	12.5	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	100 mM sodium acetate pH 5.0, 24% (w/v) polyethylene glycol (PEG) 400, 80 mM MgCl2, 3 mM ZnSO4