5JVP
The neck-linker and alpha 7 helix of Homo sapiens CENP-E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97929 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 100.609, 100.609, 107.627 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.785 - 2.100 |
R-factor | 0.239 |
Rwork | 0.237 |
R-free | 0.27390 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.427 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.786 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.114 | 0.360 |
Number of reflections | 71015 | |
<I/σ(I)> | 16.6 | 12.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 16.6 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 18% (w/v) MEPEG 2000, 175 mM Li2SO4, 100 mM MES pH 6.0, 1 mM CdCl2 |