5JVO

Crystal structure of the Arginine Repressor from the pathogenic bacterium Corynebacterium pseudotuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.458
Spacegroup name	P 21 3
Unit cell lengths	83.191, 83.191, 83.191
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 1.900
R-factor	0.1819
Rwork	0.180
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ere
RMSD bond length	0.026
RMSD bond angle	2.372
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.000	48.030	2.020
High resolution limit [Å]	1.900	5.680	1.900
Rmerge	0.079	0.032	0.899
Number of reflections	15299
<I/σ(I)>	20.81	65.66	2.15
Completeness [%]	99.9	99.4	99.5
Redundancy	10.5
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	100 mM Tris-HCl 200 mM NaCl 10% ethanol