5JVD

Tubulin-TUB092 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	104.251, 156.317, 180.614
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.457 - 2.393
R-factor	0.1707
Rwork	0.169
R-free	0.20840
RMSD bond length	0.005
RMSD bond angle	0.873
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.500	2.540
High resolution limit [Å]	2.390	2.390
Rmerge		2.064
Number of reflections	116285
<I/σ(I)>	11.6	0.95
Completeness [%]	99.6	97.7
Redundancy	7	6.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	293	6% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES/Imidazole