5JV3
The neck-linker and alpha 7 helix of Homo sapiens Eg5 fused to EB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.839, 59.502, 72.664 |
| Unit cell angles | 90.00, 100.58, 90.00 |
Refinement procedure
| Resolution | 30.622 - 2.006 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yib |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.324 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.622 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.048 | 0.414 |
| Number of reflections | 19131 | |
| <I/σ(I)> | 25.9 | 3.8 |
| Completeness [%] | 97.0 | 99.9 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 24% (w/v) PEE797, 100 mM CaCl2, 100 mM MOPS pH 7.0, 0.1% (w/v) octylglucoside |
