5JUV
STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-b-Galactopyranosyl galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.100, 106.393, 83.723 |
Unit cell angles | 90.00, 99.18, 90.00 |
Refinement procedure
Resolution | 82.650 - 2.270 |
R-factor | 0.18132 |
Rwork | 0.179 |
R-free | 0.23204 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ifp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.310 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.21) |
Phasing software | MOLREP (11.4.04) |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.650 | 2.340 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.157 | 0.584 |
Number of reflections | 46487 | |
<I/σ(I)> | 8.4 | 3.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.8 | 6.6 |
CC(1/2) | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 24% (W/V) PEG 3350, 0.1M BIS-TRIS BUFFER PH 6.0 , 0.2M LITHIUM SULPHATE, then soacked in 30mM 6-O-beta-D-Galactopyranosyl-D-galactose, and cryoprotected with 20% (W/V) PEG 400 |