5JUF

Crystal structure of the apo form of ComR from S. thermophilus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976250
Spacegroup name	C 2 2 2
Unit cell lengths	61.820, 141.680, 78.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.953 - 1.946
R-factor	0.184
Rwork	0.182
R-free	0.21630
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.753
Data reduction software	XDS
Phasing software	CRANK2
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.953	2.050
High resolution limit [Å]	1.946	1.946
Number of reflections	25537
<I/σ(I)>	12.53	2.48
Completeness [%]	99.2	96.1
Redundancy	10.9
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	291.15	20% PEG 8000, 0.1M sodium cacodylate pH=6.4, 0.2M amonium sulfate
2	VAPOR DIFFUSION, HANGING DROP	6.4	291.15	20% PEG 8000, 0.1M sodium cacodylate pH=6.4, 0.2M amonium sulfate