5JTV

USP7CD-UBL45 in complex with Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	173
Detector technology	CCD
Collection date	2014-10-29
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	113.611, 115.520, 257.822
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.954 - 3.312
R-factor	0.2402
Rwork	0.239
R-free	0.26850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nbf
RMSD bond length	0.004
RMSD bond angle	0.808
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (dev_2210)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.954	115.450	3.284
High resolution limit [Å]	3.273	15.182	3.273
Rmerge	0.127	0.036	0.853
Rmeas	0.139
Rpim	0.055
Total number of observations	323509
Number of reflections	52986
<I/σ(I)>	11.4
Completeness [%]	100.0	98.5	99.6
Redundancy	6.1	5	6.3
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.2 M K chloride, 0.05 M HEPES pH 7.5 and 35% v/v Pentaerythritol propoxylate