5JTS
Structure of a beta-1,4-mannanase, SsGH134.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.910, 53.680, 81.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.620 - 1.090 |
| R-factor | 0.14039 |
| Rwork | 0.139 |
| R-free | 0.16316 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | alpha-helical fragment |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.559 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | ACORN |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.620 | 1.120 |
| High resolution limit [Å] | 1.090 | 1.090 |
| Rmerge | 0.066 | 0.864 |
| Number of reflections | 68246 | |
| <I/σ(I)> | 12.2 | 1.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.1 | 5.8 |
| CC(1/2) | 0.532 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | The protein stock in the buffer of 20 mM sodium phosphate, pH7.2, and 200 mM NaCl is mixed 1:1 with the precipitant consist of 20 % PEG6000, 0.15-0.2 M CaCl2,and 0.1 M Na HEPES, pH7.0 |
