5JTJ

USP7CD-CTP in complex with Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	170
Detector technology	CCD
Collection date	2015-03-18
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 62 2 2
Unit cell lengths	148.060, 148.060, 179.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	74.030 - 3.321
R-factor	0.1921
Rwork	0.191
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nbf
RMSD bond length	0.009
RMSD bond angle	0.960
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.030	179.946	3.332
High resolution limit [Å]	3.321	15.410	3.321
Rmerge	0.185	0.057	1.881
Rmeas	0.190
Rpim	0.041
Total number of observations	377723
Number of reflections	17846
<I/σ(I)>	18.1
Completeness [%]	100.0	100	99.5
Redundancy	21.2	13.4	21.8
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	292	0.05 M Calcium chloride, 0.1 M MES pH 6.0 and 45% PEG 200