5JTH
Crystal structure of E67A calmodulin - CaM:RM20 analog complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.094, 57.053, 44.871 |
Unit cell angles | 90.00, 97.18, 90.00 |
Refinement procedure
Resolution | 28.000 - 1.840 |
R-factor | 0.15877 |
Rwork | 0.157 |
R-free | 0.19640 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.759 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.840 |
Number of reflections | 12634 |
<I/σ(I)> | 31.8 |
Completeness [%] | 99.8 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M Sodium Acetate pH 4.6, 25%(w/v) PEG 4000, 20mM CaCl2 |