5JTF
Crystal structure of ArsN N-acetyltransferase from Pseudomonas putida KT2440
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.017, 67.017, 206.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.644 - 2.156 |
R-factor | 0.2391 |
Rwork | 0.238 |
R-free | 0.26550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yvo |
RMSD bond length | 0.004 |
RMSD bond angle | 0.905 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.644 | 2.200 |
High resolution limit [Å] | 2.156 | 2.160 |
Number of reflections | 25849 | |
<I/σ(I)> | 6.88 | 2.91 |
Completeness [%] | 97.8 | 90.81 |
Redundancy | 5.16 | 4.31 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M Sodium acetate, 0.1 M TRIS HC (pH 8.5), 30% PEG 4000 |