5JT4
L16A mutant of cytochrome c prime from Alcaligenes xylosoxidans: Ferrous state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-02 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 53.532, 53.532, 180.938 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.250 |
Rwork | 0.149 |
R-free | 0.19120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yli |
RMSD bond length | 0.013 |
RMSD bond angle | 299.000 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.15) |
Phasing software | PHASER |
Refinement software | SHELXL (97) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.880 | 1.270 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.037 | 0.884 |
Number of reflections | 42926 | |
<I/σ(I)> | 22.8 | 2.3 |
Completeness [%] | 98.9 | 95.9 |
Redundancy | 6.3 | 6.2 |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 2.2M Ammonium sulfate 0.1 M HEPES pH 7.5 |