5JST
MBP fused MDV1 coiled coil
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.25530 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 128.515, 102.942, 79.267 |
Unit cell angles | 90.00, 102.51, 90.00 |
Refinement procedure
Resolution | 36.846 - 2.199 |
R-factor | 0.2268 |
Rwork | 0.225 |
R-free | 0.27360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dm0 2xu6 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.879 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
High resolution limit [Å] | 2.199 | 5.970 | 2.200 |
Rmerge | 0.063 | 0.036 | 0.697 |
Rmeas | 0.072 | 0.041 | 0.801 |
Rpim | 0.036 | 0.021 | 0.393 |
Total number of observations | 205521 | ||
Number of reflections | 50774 | ||
<I/σ(I)> | 15.4 | ||
Completeness [%] | 99.2 | 87.1 | 100 |
Redundancy | 4 | 3.8 | 4.1 |
CC(1/2) | 0.997 | 0.696 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 1.3~1.7 M Sodium formate, 25% PEG 3350, 0.1 M CaCl2, 0.1 M Sodium acetate/Acetic acid, pH 4.5 |