5JSI

Structure of membrane protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.85
Spacegroup name	P 1
Unit cell lengths	40.793, 56.771, 57.286
Unit cell angles	63.61, 78.48, 80.21

Refinement procedure

Resolution	50.800 - 2.000
R-factor	0.17938
Rwork	0.177
R-free	0.21999
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.751
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.051	0.501
Number of reflections	49905
<I/σ(I)>	8.64	1.43
Completeness [%]	81.9	68.6
Redundancy	1.88	1.83
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	in meso lipidic cubic phase crystallization