5JS5
Nitric oxide complex of the L16F mutant of cytochrome c prime from Alcaligenes xylosoxidans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.207, 53.207, 181.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.080 - 1.700 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yli |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.341 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.15) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.080 | 46.080 | 1.730 |
| High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
| Rmerge | 0.059 | 0.031 | 0.832 |
| Rmeas | 0.065 | ||
| Rpim | 0.026 | ||
| Total number of observations | 109533 | ||
| Number of reflections | 17754 | ||
| <I/σ(I)> | 12.7 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 6.2 | 4.6 | 6.5 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 2.2M Ammonium sulfate 0.1M Hepes pH 7.5 |
