5JRX

Crystal structure of Fe(II) CO-bound H-NOX protein from C. subterraneus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	80
Detector technology	CCD
Collection date	2015-03-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99995
Spacegroup name	C 1 2 1
Unit cell lengths	90.865, 127.205, 42.699
Unit cell angles	90.00, 95.79, 90.00

Refinement procedure

Resolution	38.187 - 1.950
R-factor	0.1881
Rwork	0.186
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u55
RMSD bond length	0.010
RMSD bond angle	1.071
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.190	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.084	0.442
Number of reflections	35087
<I/σ(I)>	14	3.6
Completeness [%]	99.9	100
Redundancy	4.7
CC(1/2)	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Anaerobically grown under Argon. 30 mg/mL protein with well condition 0.1 M LiCl, 20% PEG 3350.