5JRF
Crystal structure of the light-driven sodium pump KR2 bound with iodide ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.85 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 40.506, 83.749, 234.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 117.050 - 2.500 |
| R-factor | 0.1918 |
| Rwork | 0.190 |
| R-free | 0.22609 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.749 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.500 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.126 | 0.971 |
| Number of reflections | 26628 | |
| <I/σ(I)> | 10.25 | 1.54 |
| Completeness [%] | 99.2 | 95.5 |
| Redundancy | 6.73 | 5.5 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | in meso lipidic cubic phase crystallization |
