5JRB

Rad52(1-212) K102A/K133A/E202A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-15
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9800
Spacegroup name	P 1 21 1
Unit cell lengths	110.955, 98.516, 116.367
Unit cell angles	90.00, 91.99, 90.00

Refinement procedure

Resolution	49.258 - 2.405
R-factor	0.2261
Rwork	0.224
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kn0
RMSD bond length	0.008
RMSD bond angle	1.018
Data reduction software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	2.550
High resolution limit [Å]	2.400	2.400
Rmerge	0.095	0.633
Number of reflections	190708
<I/σ(I)>	9.2	1.9
Completeness [%]	99.6	99
Redundancy	3.5	3.5
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.05M Sodium malonate pH6.0, 5% PEG 3350