5JRA
Nitric oxide complex of the L16V mutant of cytochrome c prime from Alcaligenes xylosoxidans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 53.199, 53.199, 180.679 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.070 - 1.380 |
R-factor | 0.1637 |
Rwork | 0.163 |
R-free | 0.18580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yli |
RMSD bond length | 0.017 |
RMSD bond angle | 2.366 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.072 | 46.072 | 1.450 |
High resolution limit [Å] | 1.380 | 4.360 | 1.380 |
Rmerge | 0.030 | 0.744 | |
Number of reflections | 32339 | ||
<I/σ(I)> | 15.4 | 11.8 | 1 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 7.6 | 6.8 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 2.2M Ammonium sulfate 0.1 M HEPES pH 7.5 |