5JQW
The crystal structure of phosphoribosylaminoimidazole carboxylase ATPase subunit of Francisella tularensis subsp. tularensis SCHU S4 in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97938 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 85.230, 85.230, 103.992 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.369 - 2.060 |
R-factor | 0.1983 |
Rwork | 0.197 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mam |
RMSD bond length | 0.002 |
RMSD bond angle | 0.506 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((dev-2386_1692: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.370 | 2.100 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.090 | 0.691 |
Number of reflections | 24555 | |
<I/σ(I)> | 36.8 | 2.8 |
Completeness [%] | 99.8 | 100 |
Redundancy | 8.1 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.8M LiCl, 0.1M Tris, 10mM ADP, 32% (w/v) PEG4000 |