5JQM

Crystal Structure of Phosphatidic acid Transporter Ups1/Mdm35 Void of Bound Phospholipid from Saccharomyces Cerevisiae at 1.5 Angstroms Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	118.563, 68.290, 105.137
Unit cell angles	90.00, 102.10, 90.00

Refinement procedure

Resolution	29.560 - 1.500
R-factor	0.1656
Rwork	0.164
R-free	0.20240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jql
RMSD bond length	0.007
RMSD bond angle	1.059
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.084	0.070	0.420
Rmeas	0.100
Rpim	0.052
Total number of observations	465057
Number of reflections	129446
<I/σ(I)>	16.2
Completeness [%]	98.6	92.4	88.9
Redundancy	3.6	3.5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	289	8% Tacsimate, pH5.0, 20% PEG 3350, 200mM Sodium thiocyanate