5JQK
The Xray Crystal Structure of P. falciparum Aminopeptidase P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 146.740, 100.070, 106.660 |
Unit cell angles | 90.00, 105.37, 90.00 |
Refinement procedure
Resolution | 44.993 - 2.350 |
R-factor | 0.1904 |
Rwork | 0.189 |
R-free | 0.22560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ctz |
RMSD bond length | 0.003 |
RMSD bond angle | 0.851 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 70.750 |
High resolution limit [Å] | 2.350 |
Number of reflections | 229848 |
<I/σ(I)> | 8.2 |
Completeness [%] | 99.5 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 45% 2 methyl 2,4 pentanediol 0.1 M sodium cacodylate pH 6 |