5JQD

Antibody Fab Fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.5
Spacegroup name	P 21 21 21
Unit cell lengths	89.055, 91.577, 106.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.117 - 2.591
R-factor	0.213
Rwork	0.211
R-free	0.24160
RMSD bond length	0.005
RMSD bond angle	0.745
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.120
High resolution limit [Å]	2.590
Number of reflections	26407
<I/σ(I)>	11.16
Completeness [%]	95.0
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG 4000, 0.2 M CaCl2, 0.1 M Tris pH 8.4