5JQC

Crystal structure putative autolysin from Listeria monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	39.468, 89.323, 94.441
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.662 - 2.149
R-factor	0.1984
Rwork	0.196
R-free	0.25240
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.855
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (dev_2386)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.149
Rmerge	0.063
Number of reflections	17148
<I/σ(I)>	32.89
Completeness [%]	90.7
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.2 M Lithium sulfate, 0.1 M Tris hydrochloride, 40 % PEG 400