5JPF
Serine/Threonine phosphatase Z1 (Candida albicans) binds to inhibitor microcystin-LR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-04 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 50.665, 50.665, 201.054 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.877 - 2.399 |
| R-factor | 0.181591154876 |
| Rwork | 0.179 |
| R-free | 0.23448 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jpe |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.567 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.066 | 0.043 | 0.197 |
| Number of reflections | 12331 | ||
| <I/σ(I)> | 12.8 | ||
| Completeness [%] | 98.5 | 99.1 | 84.2 |
| Redundancy | 6.1 | 6.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 298 | 0.06 M citric acid, pH 4.1, 16% w/v PEG3350 |
