5JPE
Yeast-specific serine/threonine protein phosphatase (PPZ1) of Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-24 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 144.975, 183.727, 69.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.244 - 2.611 |
| R-factor | 0.1949 |
| Rwork | 0.194 |
| R-free | 0.22190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mov |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.719 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.660 |
| High resolution limit [Å] | 2.610 | 7.080 | 2.610 |
| Rmerge | 0.133 | 0.042 | 0.845 |
| Total number of observations | 158500 | ||
| Number of reflections | 28438 | ||
| <I/σ(I)> | 5.8 | ||
| Completeness [%] | 99.9 | 99.2 | 100 |
| Redundancy | 5.6 | 5.2 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.8 M ammonium citrate tribasic |
