5JPA
Hexameric HIV-1 CA H12Y mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.600, 90.600, 56.931 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.930 - 1.700 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h47 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.009 |
| Data reduction software | iMOSFLM (7.2.1) |
| Data scaling software | Aimless (0.4.10) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 56.930 | 56.930 | 1.730 |
| High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
| Rmerge | 0.095 | 0.063 | 0.813 |
| Rmeas | 0.105 | ||
| Rpim | 0.043 | ||
| Total number of observations | 133997 | ||
| Number of reflections | 27756 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 95.1 | 92 | 94.7 |
| Redundancy | 4.8 | 5.7 | 4.9 |
| CC(1/2) | 0.992 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 290 | PEG 550MME (12% w/v), KSCN (0.15M), 0.1M TRIS |
