5JPA
Hexameric HIV-1 CA H12Y mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9794 |
Spacegroup name | P 6 |
Unit cell lengths | 90.600, 90.600, 56.931 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.930 - 1.700 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h47 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.009 |
Data reduction software | iMOSFLM (7.2.1) |
Data scaling software | Aimless (0.4.10) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.930 | 56.930 | 1.730 |
High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
Rmerge | 0.095 | 0.063 | 0.813 |
Rmeas | 0.105 | ||
Rpim | 0.043 | ||
Total number of observations | 133997 | ||
Number of reflections | 27756 | ||
<I/σ(I)> | 9 | ||
Completeness [%] | 95.1 | 92 | 94.7 |
Redundancy | 4.8 | 5.7 | 4.9 |
CC(1/2) | 0.992 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 290 | PEG 550MME (12% w/v), KSCN (0.15M), 0.1M TRIS |