5JP7
Ferrous Leu 16 Val mutant of cytochrome c prime from Alcaligenes xylosoxidans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.309, 53.309, 180.722 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.260 |
| Rwork | 0.173 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yli |
| RMSD bond length | 0.012 |
| RMSD bond angle | 313.000 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | SHELXL (97) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.032 | 0.770 |
| Number of reflections | 43208 | |
| <I/σ(I)> | 18 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 2.2 M Ammonium sulfate 0.1 M HEPES pH 7.5 |
