5JP4
Crystal structure of S. pombe Dcp1 in complex with the decapping enhancer EDC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 39.179, 41.452, 95.626 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.813 - 2.043 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.22210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qkl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.862 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.813 | 2.150 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.110 | 1.210 |
| Number of reflections | 10434 | |
| <I/σ(I)> | 17.4 | 2.6 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 12.7 | 12.4 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1 M NaCl, 100 mM NaCitrate pH 5-6 |
