5JP2
Fcho1 Mu homology domain (Danio Rerio) with bound Eps15 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-08 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.92001 1.60 |
Spacegroup name | H 3 |
Unit cell lengths | 239.778, 239.778, 44.091 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.314 - 2.400 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.23430 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.916 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.314 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.090 | 1.401 |
Number of reflections | 35451 | |
<I/σ(I)> | 8.3 | 1 |
Completeness [%] | 96.0 | 99.1 |
Redundancy | 2.9 | 2.9 |
CC(1/2) | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 100 mM Bis-Tris propane pH, 6.0, 200 mM sodium citrate, 22% PEG 3350 and 10 mM DTT Crystals were cryopretetcted in 22% glycerol before flash cooling |