5JP2
Fcho1 Mu homology domain (Danio Rerio) with bound Eps15 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92001,1.60 |
| Spacegroup name | H 3 |
| Unit cell lengths | 239.778, 239.778, 44.091 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.314 - 2.400 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.23430 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.916 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.314 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 1.401 |
| Number of reflections | 35451 | |
| <I/σ(I)> | 8.3 | 1 |
| Completeness [%] | 96.0 | 99.1 |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 100 mM Bis-Tris propane pH, 6.0, 200 mM sodium citrate, 22% PEG 3350 and 10 mM DTT Crystals were cryopretetcted in 22% glycerol before flash cooling |
