5JP0
Bacteroides ovatus Xyloglucan PUL GH3B with bound glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-16 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.920 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.169, 104.068, 160.444 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.570 - 2.300 |
R-factor | 0.17863 |
Rwork | 0.177 |
R-free | 0.21086 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ex1 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.412 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.570 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.151 | 0.923 |
Number of reflections | 72918 | |
<I/σ(I)> | 11.4 | 2.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.1 | 8.5 |
CC(1/2) | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M sodium acetate, 15-25% PEG-3350 |