5JOF

Crystal structure of VRC03 gHVgLV antigen-binding fragment.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	90.922, 132.901, 188.575
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.461 - 3.208
R-factor	0.1976
Rwork	0.195
R-free	0.24150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3se8
RMSD bond length	0.002
RMSD bond angle	0.530
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.200
Number of reflections	36732
<I/σ(I)>	5.59
Completeness [%]	96.1
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1 M Tris pH 9, 17% PEG 8K, 0.33 M ammonium sulfate