5JO9
Structural characterization of the thermostable Bradyrhizobium japonicum d-sorbitol dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8999 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 100.879, 100.879, 88.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.834 - 2.894 |
| R-factor | 0.2713 |
| Rwork | 0.270 |
| R-free | 0.29510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nbu |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.765 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.870 | 3.070 |
| High resolution limit [Å] | 2.894 | 2.894 |
| Rmeas | 0.247 | 2.485 |
| Number of reflections | 6287 | |
| <I/σ(I)> | 12.4 | 1.14 |
| Completeness [%] | 99.5 | 98 |
| Redundancy | 10.32 | 10.17 |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.6 | 294 | 1.4 M NaH2PO4/K2HPO4 pH 5.6 |
