5JO5
Crystal structure of 10E8 gHV-gLV antigen-binding fragment.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 115.624, 65.506, 128.483 |
| Unit cell angles | 90.00, 103.99, 90.00 |
Refinement procedure
| Resolution | 38.472 - 1.700 |
| R-factor | 0.2345 |
| Rwork | 0.233 |
| R-free | 0.26260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g6f |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.687 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 296215 |
| <I/σ(I)> | 7.87 |
| Completeness [%] | 100.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% peg8000, 10% Isopropanol, imidazole, pH 6.5 |
