5JO2

Crystal structure of abscisic acid-bound abscisic acid receptor PYL3 in complex with type 2C protein phosphatase HAB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2011-12-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 21 2 21
Unit cell lengths	45.330, 75.310, 169.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.251 - 2.420
R-factor	0.2117
Rwork	0.209
R-free	0.25460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.776
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.251
High resolution limit [Å]	2.420
Number of reflections	32269
<I/σ(I)>	6.12
Completeness [%]	84.5
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277.15	0.2 M Mg(NO3)2, 0.1 M Tris-HCl pH 8.0, 28% (v/v) PEG3350