5JO1

Crystal structure of phaseic acid-bound abscisic acid receptor PYL3 in complex with type 2C protein phosphatase HAB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2013-09-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 2 21
Unit cell lengths	45.630, 75.450, 168.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.869 - 2.300
R-factor	0.2108
Rwork	0.209
R-free	0.24970
RMSD bond length	0.003
RMSD bond angle	0.737
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	68.870
High resolution limit [Å]	2.300
Number of reflections	38451
<I/σ(I)>	6.58
Completeness [%]	85.9
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.2 M Mg(NO3)2, 0.1 M Tris-HCl pH 8.0, 28% (v/v) PEG3350