5JNS
Crystal structure of human low molecular weight protein tyrosine phosphatase (LMPTP) type A complexed with phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0781 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.210, 55.090, 95.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.680 - 1.800 |
R-factor | 0.1669 |
Rwork | 0.165 |
R-free | 0.20660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jnr |
RMSD bond length | 0.030 |
RMSD bond angle | 2.613 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.9) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.090 | 55.090 | 1.770 |
High resolution limit [Å] | 1.740 | 9.020 | 1.740 |
Rmerge | 0.108 | 0.062 | 0.607 |
Number of reflections | 18220 | ||
<I/σ(I)> | 9.1 | ||
Completeness [%] | 99.8 | 96.9 | 99.9 |
Redundancy | 6.4 | 5.1 | 5.4 |
CC(1/2) | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8 | 293 | 25% PEG 3350K, 200 mM NaCl |