5JNH
Crystal Structure of cytidine monophosphate hydroxymethylase MilA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.03576 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.752, 107.752, 112.024 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.025 - 2.202 |
| R-factor | 0.1698 |
| Rwork | 0.168 |
| R-free | 0.20700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.806 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.156 | 0.072 | 0.767 |
| Rmeas | 0.160 | ||
| Rpim | 0.034 | ||
| Total number of observations | 838384 | ||
| Number of reflections | 38532 | ||
| <I/σ(I)> | 7 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 21.8 | 21.1 | 19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 289 | 15% (w/v) polyethylene glycol 3350, 0.08 M Lithium sulfate monohydrate, 0.1 M Tris HCl |
