5JNF
Crystal structure of the LgrA initiation module excluding the Asub domain: F-A-delta-sub
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 199 |
| Detector technology | CCD |
| Collection date | 2016-03-15 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 |
| Unit cell lengths | 161.311, 161.311, 139.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.793 - 2.750 |
| R-factor | 0.2486 |
| Rwork | 0.247 |
| R-free | 0.27530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5es6 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.642 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 88.400 | 88.400 | 2.600 |
| High resolution limit [Å] | 2.550 | 13.980 | 2.550 |
| Rmerge | 0.148 | 0.051 | 2.245 |
| Rmeas | 0.159 | 0.055 | 2.453 |
| Rpim | 0.059 | 0.020 | 0.976 |
| Total number of observations | 867930 | 5243 | 36367 |
| Number of reflections | 116252 | ||
| <I/σ(I)> | 8 | 22.3 | 0.8 |
| Completeness [%] | 100.0 | 97.6 | 100 |
| Redundancy | 7.5 | 7.2 | 6.3 |
| CC(1/2) | 0.997 | 0.999 | 0.169 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.3 M sodium formate, 0.1 M triNa citrate pH 5.6. |
